Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether

Authors

  • Rintaro Hoshino
  • Kazuya Murakami
  • Wahyudiono Wahyudiono
  • Siti Machmudah
  • Yuji Okita
  • Eiji Ohashi
  • Hideki Kanda
  • Motonobu Goto

DOI:

https://doi.org/10.4186/ej.2016.20.4.145 Full article

Abstract

Recently, a simple method for the extraction of lipids from wet biomass using liquefied dimethyl ether (DME) without drying, cell disruption, or heating was proposed. Here, the versatility of this method was evaluated for labyrinthula Aurantiochytrium limacinum (A. limacinum). The liquefied DME was passed through the extractor that filled by A. limacinum at different time intervals. The extraction of lipids from A. limacinum of moisture-rich microorganism was successfully achieved, the yield of lipid was 46.1 wt% of the dry weight of the sample. In comparison, the yields of lipid were 21.3 wt%, 43.6 wt% and 50.7 wt% when supercritical carbon dioxide (SCCO2), hexane-Soxhlet and Bligh-Dyer (BD) extraction methods were applied as extractants, respectively. However, the drying and cell-disruption process were required in SCCO2, hexane-Soxhlet, and BD extraction methods.

Keywords:

Aurantiochytrium limacinum, liquefied dimethyl ether, supercritical carbon dioxide, fatty acid, bio fuel, dewatering.

Affiliations

  • Rintaro Hoshino Nagoya University
  • Kazuya Murakami Nagoya University
  • Wahyudiono Wahyudiono Nagoya University
  • Siti Machmudah Sepuluh Nopember Institute of Technology
  • Yuji Okita Nippon Suisan Kaisha, Ltd.
  • Eiji Ohashi Nippon Suisan Kaisha, Ltd.
  • Hideki Kanda Nagoya University
  • Motonobu Goto Nagoya University

Corresponding author: Hideki Kanda, kanda@nuce.nagoya-u.ac.jp

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Author Biographies

  • Rintaro Hoshino

    Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

  • Kazuya Murakami

    Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

  • Wahyudiono Wahyudiono

    Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

  • Siti Machmudah

    Department of Chemical Engineering, Sepuluh Nopember Institute of Technology, Kampus ITS Sukolilo, Surabaya 60111, Indonesia

  • Yuji Okita

    Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo 192-0991, Japan

  • Eiji Ohashi

    Nippon Suisan Kaisha, Ltd., Hachioji, Tokyo 192-0991, Japan

  • Hideki Kanda

    Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan
    Japan Science and Technology Agency, Kawaguchi, Saitama 332-0012, Japan

  • Motonobu Goto

    Department of Chemical Engineering, Nagoya University, Chikusa, Nagoya 464-8603, Japan

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How to Cite

[1]
R. Hoshino et al., “Economical Wet Extraction of Lipid from labyrinthula Aurantiochytrium limacinum by Using Liquefied Dimethyl Ether”, Eng. J., vol. 20, no. 4, pp. 145–153, Aug. 2016, doi: 10.4186/ej.2016.20.4.145.

Citations

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Published

2016-08-29

Issue

Vol. 20 No. 4 (2016): Special Issue: Selected Papers from EARTH 2015

Section

Special Section for MTEC-EARTH 2015

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